5gjq

Electron Microscopy
4.5Å resolution

Structure of the human 26S proteasome bound to USP14-UbAl

Released:
Source organism: Homo sapiens
Primary publication:
An atomic structure of the human 26S proteasome.
Nat Struct Mol Biol 23 778-85 (2016)
PMID: 27428775
Related structures: EMD-9511

Function and Biology Details

Reactions catalysed:
Cleavage of peptide bonds with very broad specificity.
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 48-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126175 (preferred)
Entry contents:
34 distinct polypeptide molecules
Macromolecules (34 distinct):
Proteasome subunit beta type-6 Chains: a, o
Molecule details ›
Chains: a, o
Length: 239 amino acids
Theoretical weight: 25.38 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28072 (Residues: 1-239; Coverage: 100%)
Gene names: LMPY, PSMB6, Y
Sequence domains: Proteasome subunit
Proteasome subunit alpha type-6 Chains: B, h
Molecule details ›
Chains: B, h
Length: 246 amino acids
Theoretical weight: 27.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60900 (Residues: 1-246; Coverage: 100%)
Gene names: PROS27, PSMA6
Sequence domains:
Proteasome subunit beta type-7 Chains: b, p
Proteasome subunit alpha type-2 Chains: C, i
Proteasome subunit beta type-3 Chains: c, q
Proteasome subunit alpha type-4 Chains: D, j
Proteasome subunit beta type-2 Chains: d, r
Proteasome subunit alpha type-7 Chains: E, k
Proteasome subunit beta type-5 Chains: e, s
Proteasome subunit alpha type-5 Chains: F, l
Proteasome subunit beta type-1 Chains: f, t
Proteasome subunit alpha type-1 Chains: G, m
Proteasome subunit beta type-4 Chains: g, u
26S proteasome regulatory subunit 7 Chain: H
26S proteasome regulatory subunit 4 Chain: I
26S proteasome regulatory subunit 8 Chain: J
26S proteasome regulatory subunit 6B Chain: K
26S proteasome regulatory subunit 10B Chain: L
26S proteasome regulatory subunit 6A Chain: M
26S proteasome non-ATPase regulatory subunit 1 Chain: N
Proteasome subunit alpha type-3 Chains: X, n
26S proteasome non-ATPase regulatory subunit 13 Chain: O
26S proteasome non-ATPase regulatory subunit 12 Chain: P
26S proteasome non-ATPase regulatory subunit 11 Chain: Q
26S proteasome non-ATPase regulatory subunit 6 Chain: R
26S proteasome non-ATPase regulatory subunit 3 Chain: S
26S proteasome non-ATPase regulatory subunit 8 Chain: T
26S proteasome non-ATPase regulatory subunit 7 Chain: U
26S proteasome non-ATPase regulatory subunit 14 Chain: V
26S proteasome non-ATPase regulatory subunit 4 Chain: W
26S proteasome complex subunit SEM1 Chain: Y
26S proteasome non-ATPase regulatory subunit 2 Chain: Z
Ubiquitin carboxyl-terminal hydrolase 14 Chain: x
Ubiquitin Chain: y

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 4.5Å
Relevant EMDB volumes: EMD-9511
Expression systems:
  • Escherichia coli K-12
  • Escherichia coli