› 5gu4

X-ray diffraction
1.55Å resolution

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136297 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ricin A chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 301 amino acids
Theoretical weight: 33.49 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02879 (Residues: 36-302; Coverage: 49%)
Sequence domains: Ribosome inactivating protein
Structure domains:
Large ribosomal subunit protein P2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 9 amino acids
Theoretical weight: 1.02 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P05387 (Residues: 107-115; Coverage: 8%)
Gene names: D11S2243E, RPLP2, RPP2

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21
Unit cell:
a: 67.45Å b: 59.88Å c: 67.495Å
α: 90° β: 99.89° γ: 90°
R-values:
R R work R free
0.22 0.218 0.255
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Intracellular Transport and Cytotoxicity of the Protein Toxin Ricin.
Sowa-Rogozińska et al. (2019)
6 more

9 mentions without citation

Structures and Ribosomal Interaction of Ribosome-Inactivating Proteins.
Shi et al. (2016)
8 more