5h37

Electron Microscopy
4Å resolution

Cryo-EM structure of zika virus complexed with Fab C10 at pH 8.0

Released:

Function and Biology Details

Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 720-mer (preferred)
PDBe Complex ID:
PDB-CPX-492221 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Small envelope protein M Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 75 amino acids
Theoretical weight: 8.5 KDa
Source organism: Zika virus
UniProt:
  • Canonical: A0A024B7W1 (Residues: 216-290; Coverage: 2%)
Sequence domains: Flavivirus envelope glycoprotein M
Structure domains: Flavivirus envelope glycoprotein M-like
C10 IgG heavy chain variable region Chains: G, I, K
Molecule details ›
Chains: G, I, K
Length: 127 amino acids
Theoretical weight: 14.49 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
C10 IgG light chain variable region Chains: H, L, M
Molecule details ›
Chains: H, L, M
Length: 109 amino acids
Theoretical weight: 11.3 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-9575
Expression system: Homo sapiens