› 5hou

Solution NMR

Solution Structure of p53TAD-TAZ1

Released:
DOI: 10.2210/pdb5hou/pdb
PDB entry 5hou coloured by chain, ensemble of 20 models, front view.
PDB entry 5hou coloured by chain, ensemble of 20 models, side view.
PDB entry 5hou coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Recognition of the disordered p53 transactivation domain by the transcriptional adapter zinc finger domains of CREB-binding protein.
Krois AS, Ferreon JC, Martinez-Yamout MA, Dyson HJ, Wright PE
Proc Natl Acad Sci U S A 113 E1853-62 (2016)
PMID: 26976603

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138103 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellular tumor antigen p53; Histone lysine acetyltransferase CREBBP Chain: A
Molecule details ›
Chain: A
Length: 171 amino acids
Theoretical weight: 19.11 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04637 (Residues: 1-61, 69-69; Coverage: 16%)
  • Canonical: P45481 (Residues: 341-439; Coverage: 4%)
Gene names: Cbp, Crebbp, P53, TP53
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment: 82%
Refinement method: simulated annealing, molecular dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Application of NMR to studies of intrinsically disordered proteins.
Gibbs et al. (2017)
11 more

5 mentions without citation

p53 Proteoforms and Intrinsic Disorder: An Illustration of the Protein Structure-Function Continuum Concept.
Uversky VN. (2016)
4 more