› 5hug

X-ray diffraction
1.85Å resolution

The crystal structure of neuraminidase from A/American green-winged teal/Washington/195750/2014 influenza virus

Released:
DOI: 10.2210/pdb5hug/pdb
PDB entry 5hug coloured by chain, front view.
PDB entry 5hug coloured by chain, side view.
PDB entry 5hug coloured by chain, top view.
Source organism: Influenza A virus (A/American green-winged teal/Washington/195750/2014(H5N1))
Primary publication:
Molecular Characterizations of Surface Proteins Hemagglutinin and Neuraminidase from Recent H5Nx Avian Influenza Viruses.
Yang H, Carney PJ, Mishin VP, Guo Z, Chang JC, Wentworth DE, Gubareva LV, Stevens J
J Virol 90 5770-5784 (2016)
PMID: 27053557

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-493155 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Neuraminidase Chain: A
Molecule details ›
Chain: A
Length: 397 amino acids
Theoretical weight: 43.2 KDa
Source organism: Influenza A virus (A/American green-winged teal/Washington/195750/2014(H5N1))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: A0A0C5BL75 (Residues: 83-469; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand CA 2 x CA
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P4212
Unit cell:
a: 91.644Å b: 91.644Å c: 108.965Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.195
Expression system: Trichoplusia ni

Quick links

Citations

4 review citations

Pandemic potential of highly pathogenic avian influenza clade 2.3.4.4 A(H5) viruses.
Yamaji et al. (2020)
3 more

2 mentions without citation

Epitopes in the HA and NA of H5 and H7 avian influenza viruses that are important for antigenic drift.
Luczo et al. (2024)
1 more