5icv

X-ray diffraction
1.53Å resolution

Crystal structure of human NatF (hNaa60) bound to a bisubstrate analogue

Released:

Function and Biology Details

Reactions catalysed:
Acetyl-CoA + an N-terminal-L-methionyl-[transmembrane protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-[transmembrane protein] + CoA
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-553143 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
N-alpha-acetyltransferase 60 Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 20.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H7X0 (Residues: 5-184; Coverage: 74%)
Gene names: HAT4, NAA60, NAT15, UNQ2771/PRO7155
Sequence domains: Acetyltransferase (GNAT) family
MET-LYS-ALA-VAL-LIG Chains: C, D
Molecule details ›
Chains: C, D
Length: 4 amino acids
Theoretical weight: 449 Da
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments


Cofactor: Ligand 1XE 2 x 1XE
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P41
Unit cell:
a: 48.448Å b: 48.448Å c: 148.368Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.208
Expression system: Escherichia coli