5jpq

Electron Microscopy
7.3Å resolution

Cryo-EM structure of the 90S pre-ribosome

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + pseudouridine(1191) in yeast 18S rRNA = S-adenosyl-L-homocysteine + N(1)-methylpseudouridine(1191) in yeast 18S rRNA 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 56-mer (preferred)
PDBe Complex ID:
PDB-CPX-101206 (preferred)
Entry contents:
34 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (36 distinct):
WD40 domain proteins Chains: A, B, C, D, E, F, J, K, L, N, P, l, n
Molecule details ›
Chains: A, B, C, D, E, F, J, K, L, N, P, l, n
Length: 1290 amino acids
Theoretical weight: 109.8 KDa
Source organism: Chaetomium thermophilum
U3 small nucleolar RNA-associated protein 10 Chain: G
Molecule details ›
Chain: G
Length: 1802 amino acids
Theoretical weight: 198.51 KDa
Source organism: Chaetomium thermophilum
UTP-A oligomerization domain Chain: H
U3 small nucleolar RNA-associated protein 21 Chain: I
WD40 domain proteins Chains: M, O, m
UTP6 Chain: Q
UTP-B oligomerisation domain Chain: R
Nop domain-containing protein Chains: S, T
Large ribosomal subunit protein eL8 Chains: U, V
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase Chains: W, X
Ribosomal RNA-processing protein 9 Chain: Y
RNA 3'-terminal phosphate cyclase-like protein Chain: Z
Ribosome biogenesis protein BMS1 Chain: a
Imp3 Chain: b
U3 small nucleolar ribonucleoprotein protein IMP4 Chain: c
PIN domain-containing protein Chain: d
Ribosomal RNA small subunit methyltransferase NEP1 Chains: e, f
KRR1 small subunit processome component Chains: g, h
RNA cytidine acetyltransferase Chains: i, j
Utp30 Chain: k
Small ribosomal subunit protein eS1 Chain: o
Small ribosomal subunit protein eS4 Chain: p
Small ribosomal subunit protein uS7 Chain: q
40S ribosomal protein S6 Chain: r
40S ribosomal protein S7 Chain: s
40S ribosomal protein S8 Chain: t
Small ribosomal subunit protein uS4A Chain: u
Small ribosomal subunit protein uS15 Chain: v
Small ribosomal subunit protein uS11A Chain: w
Small ribosomal subunit protein uS9A Chain: x
Small ribosomal subunit protein uS17 N-terminal domain-containing protein Chain: y
Small ribosomal subunit protein uS8A Chain: z
40S ribosomal protein S24 Chain: 0
Small ribosomal subunit protein eS28A Chain: 1
18S Ribosomal RNA Chain: 2
U3 RNA Chain: 3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.3Å
Relevant EMDB volumes: EMD-8143