› 5jz6

X-ray diffraction
2.35Å resolution

Aspartyl/Asparaginyl beta-hydroxylase (AspH)oxygenase and TPR domains in complex with manganese and L-malate

Released:
DOI: 10.2210/pdb5jz6/pdb
PDB entry 5jz6 coloured by chain, front view.
PDB entry 5jz6 coloured by chain, side view.
PDB entry 5jz6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Aspartate/asparagine-β-hydroxylase crystal structures reveal an unexpected epidermal growth factor-like domain substrate disulfide pattern.
Pfeffer I, Brewitz L, Krojer T, Jensen SA, Kochan GT, Kershaw NJ, Hewitson KS, McNeill LA, Kramer H, Münzel M, Hopkinson RJ, Oppermann U, Handford PA, McDonough MA, Schofield CJ
OpenAccess logo Nat Commun 10 4910 (2019)
PMID: 31659163

Function and Biology Details

Reaction catalysed: 
Peptide L-aspartate + 2-oxoglutarate + O(2) = peptide 3-hydroxy-L-aspartate + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartyl/asparaginyl beta-hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 429 amino acids
Theoretical weight: 49.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12797 (Residues: 330-758; Coverage: 57%)
Gene names: ASPH, BAH
Sequence domains:
Structure domains: B-lactam Antibiotic, Isopenicillin N Synthase; Chain

Ligands and Environments

3 bound ligands:
Ligand MN 1 x MN
Ligand GOL 4 x GOL
Ligand LMR 1 x LMR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 49.019Å b: 70.641Å c: 172.541Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Aspartate β-hydroxylase as a target for cancer therapy.
Kanwal et al. (2020)
3 more