› 5kle

X-ray diffraction
1.5Å resolution

Structure of CBM_E1, a novel carbohydrate-binding module found by sugar cane soil metagenome, complexed with cellopentaose

Released:
DOI: 10.2210/pdb5kle/pdb
PDB entry 5kle coloured by chain, front view.
PDB entry 5kle coloured by chain, side view.
PDB entry 5kle coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
A Novel Carbohydrate-binding Module from Sugar Cane Soil Metagenome Featuring Unique Structural and Carbohydrate Affinity Properties.
Campos BM, Liberato MV, Alvarez TM, Zanphorlin LM, Ematsu GC, Barud H, Polikarpov I, Ruller R, Gilbert HJ, Zeri AC, Squina FM
J Biol Chem 291 23734-23743 (2016)
PMID: 27621314

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Carbohydrate binding module E1 Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 10.01 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0R5P8X1 (Residues: 1-93; Coverage: 100%)

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: I213
Unit cell:
a: 88.812Å b: 88.812Å c: 88.812Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.183
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Advances in molecular engineering of carbohydrate-binding modules.
Armenta et al. (2017)
7 other articles

1 mention without citation

A Novel Carbohydrate-binding Module from Sugar Cane Soil Metagenome Featuring Unique Structural and Carbohydrate Affinity Properties.
Campos et al. (2016)