› 5kwq

X-ray diffraction
2.8Å resolution

Two Tandem RRM Domains of FBP-Interacting Repressor (FIR), also Known as PUF60

Released:
DOI: 10.2210/pdb5kwq/pdb
PDB entry 5kwq coloured by chain, front view.
PDB entry 5kwq coloured by chain, side view.
PDB entry 5kwq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unraveling the mechanism of recognition of the 3' splice site of the adenovirus major late promoter intron by the alternative splicing factor PUF60.
Hsiao HT, Crichlow GV, Murphy JW, Folta-Stogniew EJ, Lolis EJ, Braddock DT
OpenAccess logo PLoS One 15 e0242725 (2020)
PMID: 33253191

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-577446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(U)-binding-splicing factor PUF60 Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 23.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UHX1 (Residues: 118-316; Coverage: 36%)
Gene names: FIR, PUF60, ROBPI, SIAHBP1
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P31
Unit cell:
a: 61.831Å b: 61.831Å c: 80.412Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.239 0.239 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Structural insights into a regulatory mechanism of FIR RRM1-FUSE interaction.
Ni et al. (2023)
1 more

1 mention without citation

Unraveling the mechanism of recognition of the 3' splice site of the adenovirus major late promoter intron by the alternative splicing factor PUF60.
Hsiao et al. (2020)