› 5lym

X-ray diffraction
1.8Å resolution

STUDIES OF MONOCLINIC HEN EGG WHITE LYSOZYME. IV. X-RAY REFINEMENT AT 1.8 ANGSTROM RESOLUTION AND A COMPARISON OF THE VARIABLE REGIONS IN THE POLYMORPHIC FORMS

Released:
DOI: 10.2210/pdb5lym/pdb
PDB entry 5lym coloured by chain, front view.
PDB entry 5lym coloured by chain, side view.
PDB entry 5lym coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Studies of monoclinic hen egg-white lysozyme. IV. X-ray refinement at 1.8 A resolution and a comparison of the variable regions in the polymorphic forms.
Rao ST, Sundaralingam M
Acta Crystallogr D Biol Crystallogr 52 170-5 (1996)
PMID: 15299739

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:
Ligand NO3 6 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 28Å b: 62.5Å c: 60.9Å
α: 90° β: 90.8° γ: 90°
R-values:
R R work R free
0.189 0.189 0.264

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Citations

3 review citations

Design of a confined environment using protein cages and crystals for the development of biohybrid materials.
Abe et al. (2016)
2 more

3 mentions without citation

The use of trimethylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents for macromolecular crystallography.
Marshall et al. (2012)
2 more