› 5m6x

X-ray diffraction
2.4Å resolution

Crystal Structure of human RhoGAP mutated in its arginine finger (R85A) in complex with RhoA.GDP.MgF3- human

Released:
DOI: 10.2210/pdb5m6x/pdb
PDB entry 5m6x coloured by chain, front view.
PDB entry 5m6x coloured by chain, side view.
PDB entry 5m6x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Assessing the Influence of Mutation on GTPase Transition States by Using X-ray Crystallography, 19 F NMR, and DFT Approaches.
Jin Y, Molt RW, Pellegrini E, Cliff MJ, Bowler MW, Richards NGJ, Blackburn GM, Waltho JP
OpenAccess logo Angew Chem Int Ed Engl 56 9732-9735 (2017)
PMID: 28498638

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-548622 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho GTPase-activating protein 1 Chains: A, H
Molecule details ›
Chains: A, H
Length: 240 amino acids
Theoretical weight: 27.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07960 (Residues: 198-437; Coverage: 55%)
Gene names: ARHGAP1, CDC42GAP, RHOGAP1
Sequence domains: RhoGAP domain
Structure domains: Rho GTPase activation protein
Transforming protein RhoA Chains: B, I
Molecule details ›
Chains: B, I
Length: 192 amino acids
Theoretical weight: 21.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61586 (Residues: 2-193; Coverage: 100%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand GDP 2 x GDP
Ligand MGF 2 x MGF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 73.47Å b: 66.69Å c: 76.89Å
α: 90° β: 95.41° γ: 90°
R-values:
R R work R free
0.227 0.224 0.272
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Spectroscopic glimpses of the transition state of ATP hydrolysis trapped in a bacterial DnaB helicase.
Malär et al. (2021)
1 more

1 mention without citation

Assessing the Influence of Mutation on GTPase Transition States by Using X-ray Crystallography, 19 F NMR, and DFT Approaches.
Jin et al. (2017)