› 5m7c

X-ray diffraction
1.6Å resolution

Blood group synthase AAGlyB in complex with UDP and cryoprotected with PEG 3350

Released:
DOI: 10.2210/pdb5m7c/pdb
PDB entry 5m7c coloured by chain, front view.
PDB entry 5m7c coloured by chain, side view.
PDB entry 5m7c coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Rocha J, Royant A

Function and Biology Details

Reactions catalysed: 
UDP-alpha-D-galactose + alpha-L-fucosyl-(1->2)-D-galactosyl-R = UDP + alpha-D-galactosyl-(1->3)-(alpha-L-fucosyl-(1->2))-D-galactosyl-R
UDP-N-acetyl-alpha-beta-D-galactosamine + glycoprotein-alpha-L-fucosyl-(1->2)-D-galactose = UDP + glycoprotein-N-acetyl-alpha-D-galactosaminyl-(1->3)-(alpha-L-fucosyl-(1->2))-beta-D-galactose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147726 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 34.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P16442 (Residues: 64-354; Coverage: 82%)
Gene name: ABO
Sequence domains: Glycosyltransferase family 6
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand UDP 2 x UDP
Ligand PEG 2 x PEG
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 52.73Å b: 77.768Å c: 79.995Å
α: 90° β: 109.19° γ: 90°
R-values:
R R work R free
0.152 0.151 0.18
Expression system: Escherichia coli

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