› 5mds

X-ray diffraction
2.6Å resolution

Crystal structure of outer membrane expressed Chitoporin VhChip from Vibrio harveyi in complex with chitotetraose

Released:
DOI: 10.2210/pdb5mds/pdb
PDB entry 5mds coloured by chain, front view.
PDB entry 5mds coloured by chain, side view.
PDB entry 5mds coloured by chain, top view.
Source organism: Vibrio harveyi
Primary publication:
Structural basis for chitin acquisition by marine Vibrio species.
Aunkham A, Zahn M, Kesireddy A, Pothula KR, Schulte A, Baslé A, Kleinekathöfer U, Suginta W, van den Berg B
OpenAccess logo Nat Commun 9 220 (2018)
PMID: 29335469

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-516748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Porin domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 350 amino acids
Theoretical weight: 38.54 KDa
Source organism: Vibrio harveyi
Expression system: Escherichia coli
UniProt:
  • Canonical: L0RVU0 (Residues: 26-375; Coverage: 100%)
Gene name: chiP
Sequence domains: Gram-negative porin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 250.01Å b: 145.047Å c: 55.158Å
α: 90° β: 94.8° γ: 90°
R-values:
R R work R free
0.222 0.221 0.247
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Role of the lipid bilayer in outer membrane protein folding in Gram-negative bacteria.
Horne et al. (2020)
1 more

4 mentions without citation

Structural basis for chitin acquisition by marine Vibrio species.
Aunkham et al. (2018)
3 more