› 5o0w

X-ray diffraction
2.57Å resolution

Crystal structure of the complex between Nb474 and Trypanosoma congolense fructose-1,6-bisphosphate aldolase

Released:
DOI: 10.2210/pdb5o0w/pdb
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PDB entry 5o0w coloured by chain, front view.
PDB entry 5o0w coloured by chain, side view.
PDB entry 5o0w coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the high specificity of a Trypanosoma congolense immunoassay targeting glycosomal aldolase.
Pinto J, Odongo S, Lee F, Gaspariunaite V, Muyldermans S, Magez S, Sterckx YG
OpenAccess logo PLoS Negl Trop Dis 11 e0005932 (2017)
PMID: 28915239

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207867 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fructose-bisphosphate aldolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 387 amino acids
Theoretical weight: 42.71 KDa
Source organism: Trypanosoma congolense IL3000
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G0UWE7 (Residues: 1-372; Coverage: 100%)
Gene name: TCIL3000_10_4760
Sequence domains: Fructose-bisphosphate aldolase class-I
Structure domains: Aldolase class I
Nb474 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 143 amino acids
Theoretical weight: 15.8 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21212
Unit cell:
a: 120.82Å b: 188.87Å c: 126.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.222
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

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Citations

5 review citations

NANOBODIES®: A Review of Diagnostic and Therapeutic Applications
Jin et al. (2023)
4 more

2 mentions without citation

Structural basis for the high specificity of a Trypanosoma congolense immunoassay targeting glycosomal aldolase.
Pinto et al. (2017)
1 more

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