› 5o1j

X-ray diffraction
1.9Å resolution

Lytic transglycosylase in action

Released:
DOI: 10.2210/pdb5o1j/pdb
PDB entry 5o1j coloured by chain, front view.
PDB entry 5o1j coloured by chain, side view.
PDB entry 5o1j coloured by chain, top view.
Source organism: Neisseria meningitidis MC58
Primary publication:
A step-by-step
Williams AH, Wheeler R, Rateau L, Malosse C, Chamot-Rooke J, Haouz A, Taha MK, Boneca IG
J Biol Chem 293 6000-6010 (2018)
PMID: 29483188

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-575442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Soluble lytic murein transglycosylase Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 64.11 KDa
Source organism: Neisseria meningitidis MC58
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9JXP1 (Residues: 38-616; Coverage: 97%)
Gene name: NMB1949
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: Z4S, NAG
Carbohydrate polymer
1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 68.135Å b: 69.122Å c: 143.321Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.201 0.249
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Cell-Wall Recycling of the Gram-Negative Bacteria and the Nexus to Antibiotic Resistance.
Dik et al. (2018)
1 more

1 mention without citation

A step-by-step in crystallo guide to bond cleavage and 1,6-anhydro-sugar product synthesis by a peptidoglycan-degrading lytic transglycosylase.
Williams et al. (2018)