› 5ohf

X-ray diffraction
1.8Å resolution

Globin sensor domain of AfGcHK (FeIII form) in complex with cyanide, partially reduced

Released:
DOI: 10.2210/pdb5ohf/pdb
PDB entry 5ohf coloured by chain, front view.
PDB entry 5ohf coloured by chain, side view.
PDB entry 5ohf coloured by chain, top view.
Source organism: Anaeromyxobacter sp. Fw109-5
Primary publication:
Coordination and redox state-dependent structural changes of the heme-based oxygen sensor AfGcHK associated with intraprotein signal transduction.
Stranava M, Man P, Skálová T, Kolenko P, Blaha J, Fojtikova V, Martínek V, Dohnálek J, Lengalova A, Rosůlek M, Shimizu T, Martínková M
J Biol Chem 292 20921-20935 (2017)
PMID: 29092908

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Globin-coupled histidine kinase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 161 amino acids
Theoretical weight: 18.37 KDa
Source organism: Anaeromyxobacter sp. Fw109-5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A7HD43 (Residues: 2-161; Coverage: 42%)
Gene names: Anae109_2438, gchK
Sequence domains: Protoglobin

Ligands and Environments


Cofactor: Ligand HEM 8 x HEM
3 bound ligands:
Ligand CYN 8 x CYN
Ligand CL 5 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P41212
Unit cell:
a: 77.685Å b: 77.685Å c: 441.238Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

The multifaceted role of iron in renal health and disease.
van Swelm et al. (2020)
7 other articles

2 mentions without citation

Coordination and redox state-dependent structural changes of the heme-based oxygen sensor AfGcHK associated with intraprotein signal transduction.
Stranava et al. (2017)
1 more