› 5oho

X-ray diffraction
1.6Å resolution

Crystal structure of the KOWx-KOW4 domain of human DSIF

Released:
DOI: 10.2210/pdb5oho/pdb
PDB entry 5oho coloured by chain, front view.
PDB entry 5oho coloured by chain, side view.
PDB entry 5oho coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a transcribing RNA polymerase II-DSIF complex reveals a multidentate DNA-RNA clamp.
Bernecky C, Plitzko JM, Cramer P
Nat Struct Mol Biol 24 809-815 (2017)
PMID: 28892040

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-517186 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription elongation factor SPT5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 113 amino acids
Theoretical weight: 12.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00267 (Residues: 536-646; Coverage: 10%)
Gene names: SPT5, SPT5H, SUPT5H

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 50.286Å b: 54.403Å c: 73.065Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.174 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

Eukaryotic core promoters and the functional basis of transcription initiation.
Haberle et al. (2018)
24 more

2 mentions without citation

NusG, an Ancient Yet Rapidly Evolving Transcription Factor.
Wang et al. (2020)
1 more