› 5oju

X-ray diffraction
2.17Å resolution

Crystal structure of Mycolicibacterium hassiacum glucosylglycerate hydrolase (MhGgH) E419A variant in complex with glucosylglycerate

Released:
DOI: 10.2210/pdb5oju/pdb
PDB entry 5oju coloured by chain, front view.
PDB entry 5oju coloured by chain, side view.
PDB entry 5oju coloured by chain, top view.
Source organism: Mycolicibacterium hassiacum DSM 44199
Primary publication:
The structural characterization of a glucosylglycerate hydrolase provides insights into the molecular mechanism of mycobacterial recovery from nitrogen starvation.
Cereija TB, Alarico S, Lourenço EC, Manso JA, Ventura MR, Empadinhas N, Macedo-Ribeiro S, Pereira PJB
OpenAccess logo IUCrJ 6 572-585 (2019)
PMID: 31316802

Function and Biology Details

Reaction catalysed: 
2-O-(alpha-D-glucopyranosyl)-D-glycerate + H(2)O = D-glucopyranose + D-glycerate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125567 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosylglycerate hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 448 amino acids
Theoretical weight: 50.9 KDa
Source organism: Mycolicibacterium hassiacum DSM 44199
Expression system: Escherichia coli
UniProt:
  • Canonical: K5BDL0 (Residues: 1-446; Coverage: 100%)
Gene names: C731_0006, ggh
Sequence domains: Mannosylglycerate hydrolase MGH1-like glycoside hydrolase domain

Ligands and Environments

3 bound ligands:
Ligand 9WN 2 x 9WN
Ligand GOL 9 x GOL
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P21212
Unit cell:
a: 86.097Å b: 159.073Å c: 88.406Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.198
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Crystal structure of β-L-arabinobiosidase belonging to glycoside hydrolase family 121.
Saito et al. (2020)
1 more

1 mention without citation

The structural characterization of a glucosylglycerate hydrolase provides insights into the molecular mechanism of mycobacterial recovery from nitrogen starvation.
Cereija et al. (2019)