› 5oxh

X-ray diffraction
1.6Å resolution

C-terminally retracted ubiquitin T66V/L67N mutant

Released:
DOI: 10.2210/pdb5oxh/pdb
PDB entry 5oxh coloured by chain, front view.
PDB entry 5oxh coloured by chain, side view.
PDB entry 5oxh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An invisible ubiquitin conformation is required for efficient phosphorylation by PINK1.
Gladkova C, Schubert AF, Wagstaff JL, Pruneda JN, Freund SM, Komander D
OpenAccess logo EMBO J 36 3555-3572 (2017)
PMID: 29133469

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P3221
Unit cell:
a: 49.77Å b: 49.77Å c: 89.27Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.192 0.223
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Building and decoding ubiquitin chains for mitophagy.
Harper et al. (2018)
5 more

3 mentions without citation

Structure of PINK1 in complex with its substrate ubiquitin.
Schubert et al. (2017)
2 more