› 5qqm

X-ray diffraction
2.02Å resolution

PanDDA analysis group deposition -- Crystal Structure of Kalirin/Rac1 in complex with MolPort-009-565-325

Released:
DOI: 10.2210/pdb5qqm/pdb
PDB entry 5qqm coloured by chain, front view.
PDB entry 5qqm coloured by chain, side view.
PDB entry 5qqm coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Gray JL, Krojer T, Talon R, Douangamath A, Jimenez Antunez C, Bountra C, Arrowsmith CH, Edwards A, Brennan PE, von Delft F

Function and Biology Details

Reactions catalysed: 
GTP + H(2)O = GDP + phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-520991 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related C3 botulinum toxin substrate 1 Chain: A
Molecule details ›
Chain: A
Length: 178 amino acids
Theoretical weight: 19.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63000 (Residues: 1-177; Coverage: 92%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Kalirin Chain: B
Molecule details ›
Chain: B
Length: 182 amino acids
Theoretical weight: 21.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60229 (Residues: 1280-1459; Coverage: 6%)
Gene names: DUET, DUO, HAPIP, KALRN, TRAD
Sequence domains: RhoGEF domain

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand N74 1 x N74
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P6522
Unit cell:
a: 62.498Å b: 62.498Å c: 342.286Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.211 0.273
Expression system: Escherichia coli

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