› 5rsa

Hybrid

COMPARISON OF TWO INDEPENDENTLY REFINED MODELS OF RIBONUCLEASE-A

Released:
DOI: 10.2210/pdb5rsa/pdb
PDB entry 5rsa coloured by chain, front view.
PDB entry 5rsa coloured by chain, side view.
PDB entry 5rsa coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Comparison of Two Independently Refined Models of Ribonuclease-A
Wlodawer A, Borkakoti N, Moss DS, Howlin B
Acta Crystallogr., B 42 379- (1986)

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand DOD 128 x DOD
No modified residues

Experiments and Validation Details

Experimental Method: Neutron Diffraction, X-ray diffraction
Spacegroup: P21
Unit cell:
a: 30.18Å b: 38.4Å c: 53.32Å
α: 90° β: 105.85° γ: 90°
R-values:
R R work R free
0.159 not available not available
Expression system: Not provided

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Citations

1 mention without citation

A novel energy-based stochastic method for positioning polar protons in protein structures from X-rays.
Glick et al. (2000)