› 5t7k

X-ray diffraction
1.3Å resolution

X-ray crystal structure of AA13 LPMO

Released:
DOI: 10.2210/pdb5t7k/pdb
PDB entry 5t7k coloured by chain, front view.
PDB entry 5t7k coloured by chain, side view.
PDB entry 5t7k coloured by chain, top view.
Source organism: Aspergillus oryzae RIB40
Primary publication:
Learning from oligosaccharide soaks of crystals of an AA13 lytic polysaccharide monooxygenase: crystal packing, ligand binding and active-site disorder.
Frandsen KE, Poulsen JC, Tovborg M, Johansen KS, Lo Leggio L
Acta Crystallogr D Struct Biol 73 64-76 (2017)
PMID: 28045386

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174063 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inactive AA13 family lytic polysaccharide monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 25.69 KDa
Source organism: Aspergillus oryzae RIB40
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: Q2U8Y3 (Residues: 47-279; Coverage: 84%)
Gene names: AA13, AO090701000246
Sequence domains: Lytic polysaccharide mono-oxygenase, cellulose-degrading

Ligands and Environments

2 bound ligands:
Ligand NAG 1 x NAG
Ligand ZN 4 x ZN
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P212121
Unit cell:
a: 45.56Å b: 59.08Å c: 71.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.093 0.091 0.135
Expression system: Aspergillus oryzae

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Citations

2 review citations

Oxygen Activation by Cu LPMOs in Recalcitrant Carbohydrate Polysaccharide Conversion to Monomer Sugars.
Meier et al. (2018)
1 more