› 5tsh

X-ray diffraction
2.3Å resolution

PilB from Geobacter metallireducens bound to AMP-PNP

Released:
DOI: 10.2210/pdb5tsh/pdb
PDB entry 5tsh coloured by chain, front view.
PDB entry 5tsh coloured by chain, side view.
PDB entry 5tsh coloured by chain, top view.
Source organism: Geobacter metallireducens GS-15
Primary publication:
The molecular mechanism of the type IVa pilus motors.
McCallum M, Tammam S, Khan A, Burrows LL, Howell PL
OpenAccess logo Nat Commun 8 15091 (2017)
PMID: 28474682

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial type II secretion system protein E domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 588 amino acids
Theoretical weight: 64.89 KDa
Source organism: Geobacter metallireducens GS-15
Expression system: Escherichia coli
UniProt:
  • Canonical: Q39VU7 (Residues: 1-568; Coverage: 100%)
Gene names: Gmet_1393, pilB
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 6 x ZN
Ligand ANP 4 x ANP
Ligand MG 2 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P1
Unit cell:
a: 74.32Å b: 99.96Å c: 109.79Å
α: 112.99° β: 106.6° γ: 88.91°
R-values:
R R work R free
0.184 0.184 0.218
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Type IV pili: dynamics, biophysics and functional consequences.
Craig et al. (2019)
10 more

5 mentions without citation

Mechanism of assembly of type 4 filaments: everything you always wanted to know (but were afraid to ask).
Pelicic V. (2023)
4 more