› 5ubf

X-ray diffraction
2.6Å resolution

Crystal structure of the RctB domains 2-3, RctB-155-483

Released:
DOI: 10.2210/pdb5ubf/pdb
PDB entry 5ubf coloured by chain, front view.
PDB entry 5ubf coloured by chain, side view.
PDB entry 5ubf coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
The replication initiator of the cholera pathogen's second chromosome shows structural similarity to plasmid initiators.
Orlova N, Gerding M, Ivashkiv O, Olinares PDB, Chait BT, Waldor MK, Jeruzalmi D
OpenAccess logo Nucleic Acids Res 45 3724-3737 (2017)
PMID: 28031373

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-575734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RctB replication initiator protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 336 amino acids
Theoretical weight: 39.86 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KNG2 (Residues: 155-483; Coverage: 50%)
Gene name: VC_A0002
Sequence domains: Vibrionales, replication initiator protein RctB, central region

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: R3
Unit cell:
a: 128.535Å b: 128.535Å c: 127.775Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.235 0.276
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The Divided Bacterial Genome: Structure, Function, and Evolution.
diCenzo et al. (2017)
5 more

1 mention without citation

The replication enhancer crtS depends on transcription factor Lrp for modulating binding of initiator RctB to ori2 of Vibrio cholerae.
Doan et al. (2024)