› 5ue3

X-ray diffraction
1.6Å resolution

Function and Biology Details

Reaction catalysed: 
Cleavage of gelatin types I and V and collagen types IV and V.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-537717 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
67 kDa matrix metalloproteinase-9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 236 amino acids
Theoretical weight: 26.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14780 (Residues: 35-445; Coverage: 34%)
Gene names: CLG4B, MMP9
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand CA 5 x CA
Ligand SO4 6 x SO4
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 90.3Å b: 73.2Å c: 77.5Å
α: 90° β: 106.3° γ: 90°
R-values:
R R work R free
0.158 0.154 0.184
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Targeting Tumor Microenvironment for Cancer Therapy.
Roma-Rodrigues et al. (2019)
24 more

7 mentions without citation

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
6 more