› 5uw0

X-ray diffraction
2.73Å resolution

Activated state yGsy2p in complex with UDP-2-fluoro-2-deoxy-glucose

Released:
DOI: 10.2210/pdb5uw0/pdb
PDB entry 5uw0 coloured by chain, front view.
PDB entry 5uw0 coloured by chain, side view.
PDB entry 5uw0 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Entry authors: Mahalingan KK, Hurley TD

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151009 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycogen [starch] synthase isoform 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 720 amino acids
Theoretical weight: 81.82 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P27472 (Residues: 1-700; Coverage: 99%)
Gene names: GSY2, L8479.8, YLR258W
Sequence domains: Glycogen synthase

Ligands and Environments

3 bound ligands:
Ligand UDP 3 x UDP
Ligand G6P 4 x G6P
Ligand U2F 1 x U2F
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I222
Unit cell:
a: 193.516Å b: 204.705Å c: 206.514Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.224
Expression system: Escherichia coli

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