› 5wkt

X-ray diffraction
3.2Å resolution

3.2-Angstrom In situ Mylar structure of bovine opsin at 100 K

Released:
DOI: 10.2210/pdb5wkt/pdb
PDB entry 5wkt coloured by chain, front view.
PDB entry 5wkt coloured by chain, side view.
PDB entry 5wkt coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
High-throughput in situ X-ray screening of and data collection from protein crystals at room temperature and under cryogenic conditions.
Broecker J, Morizumi T, Ou WL, Klingel V, Kuo A, Kissick DJ, Ishchenko A, Lee MY, Xu S, Makarov O, Cherezov V, Ogata CM, Ernst OP
Nat Protoc 13 260-292 (2018)
PMID: 29300389

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136074 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 39.03 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02699 (Residues: 1-348; Coverage: 100%)
Gene name: RHO
Sequence domains:
Structure domains: Rhodopsin 7-helix transmembrane proteins
Transducin Galpha peptide Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.26 KDa
Source organism: Bos taurus
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand BOG 3 x BOG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: R32
Unit cell:
a: 242.043Å b: 242.043Å c: 110.637Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.275 0.274 0.295
Expression system: Not provided

Quick links

Citations

7 review citations

Protein X-ray Crystallography and Drug Discovery.
Maveyraud et al. (2020)
6 more

1 mention without citation

In Silico Studies Targeting G-protein Coupled Receptors for Drug Research Against Parkinson's Disease.
Lemos et al. (2018)