› 5wtq

X-ray diffraction
1.9Å resolution

Crystal structure of human proteasome-assembling chaperone PAC4

Released:
DOI: 10.2210/pdb5wtq/pdb
PDB entry 5wtq coloured by chain, front view.
PDB entry 5wtq coloured by chain, side view.
PDB entry 5wtq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human proteasome assembly chaperone PAC4 involved in proteasome formation.
Kurimoto E, Satoh T, Ito Y, Ishihara E, Okamoto K, Yagi-Utsumi M, Tanaka K, Kato K
Protein Sci 26 1080-1085 (2017)
PMID: 28263418

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177330 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteasome assembly chaperone 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 126 amino acids
Theoretical weight: 14.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5JS54 (Residues: 1-123; Coverage: 100%)
Gene names: C6orf86, PAC4, PSMG4
Sequence domains: Proteasome assembly chaperone 4

Ligands and Environments

2 bound ligands:
Ligand NI 4 x NI
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: F222
Unit cell:
a: 72.548Å b: 152.779Å c: 212.911Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Structural insights on the dynamics of proteasome formation.
Kato et al. (2018)
1 more

2 mentions without citation

Molecular and Structural Basis of the Proteasome α Subunit Assembly Mechanism Mediated by the Proteasome-Assembling Chaperone PAC3-PAC4 Heterodimer.
Satoh et al. (2019)
1 more