› 5x2m

X-ray diffraction
2.21Å resolution

Crystal structure of the medaka fish taste receptor T1r2a-T1r3 ligand binding domains in complex with L-glutamine

Released:
DOI: 10.2210/pdb5x2m/pdb
PDB entry 5x2m coloured by chain, front view.
PDB entry 5x2m coloured by chain, side view.
PDB entry 5x2m coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for perception of diverse chemical substances by T1r taste receptors.
Nuemket N, Yasui N, Kusakabe Y, Nomura Y, Atsumi N, Akiyama S, Nango E, Kato Y, Kaneko MK, Takagi J, Hosotani M, Yamashita A
Nat Commun 8 15530 (2017)
PMID: 28534491

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-207366 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
G-protein coupled receptors family 3 profile domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 461 amino acids
Theoretical weight: 51.47 KDa
Source organism: Oryzias latipes
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: A0A173M0G2 (Residues: 12-466; Coverage: 57%)
Gene name: TAS1R2a
Sequence domains: Receptor family ligand binding region
Taste receptor type 1 member 3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 478 amino acids
Theoretical weight: 53.13 KDa
Source organism: Oryzias latipes
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: A0A173M094 (Residues: 12-483; Coverage: 57%)
Gene name: TAS1R3
Sequence domains: Receptor family ligand binding region
Fab16A Heavy chain Chains: H, J
Molecule details ›
Chains: H, J
Length: 225 amino acids
Theoretical weight: 24.18 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Fab16A Light chain Chains: K, L
Molecule details ›
Chains: K, L
Length: 217 amino acids
Theoretical weight: 23.9 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

5 bound ligands:
Ligand NAG 27 x NAG
Ligand GLN 4 x GLN
Ligand NA 3 x NA
Ligand CL 2 x CL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 99.656Å b: 116.355Å c: 129.476Å
α: 90° β: 92.2° γ: 90°
R-values:
R R work R free
0.176 0.173 0.227
Expression systems:
  • Drosophila melanogaster
  • Mus musculus

Quick links

Citations

12 review citations

Structural insights into G-protein-coupled receptor allostery.
Thal et al. (2018)
11 more

8 mentions without citation

Structural basis for perception of diverse chemical substances by T1r taste receptors.
Nuemket et al. (2017)
7 more