› 5x5h

X-ray diffraction
1.51Å resolution

Crystal structure of metB from Corynebacterium glutamicum

Released:
DOI: 10.2210/pdb5x5h/pdb
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Source organism: Corynebacterium glutamicum ATCC 13032
Primary publication:
Structural Insights into Substrate Specificity of Cystathionine γ-Synthase from Corynebacterium glutamicum.
Sagong HY, Kim KJ
J Agric Food Chem 65 6002-6008 (2017)
PMID: 28675039

Function and Biology Details

Reaction catalysed: 
O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181299 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine beta-lyases/cystathionine gamma-synthases Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 42.53 KDa
Source organism: Corynebacterium glutamicum ATCC 13032
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q79VD9 (Residues: 1-386; Coverage: 100%)
Gene name: Cgl2446
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
2 bound ligands:
Ligand GOL 3 x GOL
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: F222
Unit cell:
a: 58.574Å b: 149.851Å c: 161.858Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.148 0.172
Expression system: Escherichia coli BL21

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Citations

1 review citation

Molecular targets for antifungals in amino acid and protein biosynthetic pathways.
Kuplińska et al. (2021)
2 other articles

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