› 5y11

X-ray diffraction
2.1Å resolution

SFTSV GN with neutralizing antibody MAb4-5

Released:
DOI: 10.2210/pdb5y11/pdb
PDB entry 5y11 coloured by chain, front view.
PDB entry 5y11 coloured by chain, side view.
PDB entry 5y11 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of phlebovirus glycoprotein Gn and identification of a neutralizing antibody epitope.
Wu Y, Zhu Y, Gao F, Jiao Y, Oladejo BO, Chai Y, Bi Y, Lu S, Dong M, Zhang C, Huang G, Wong G, Li N, Zhang Y, Li Y, Feng WH, Shi Y, Liang M, Zhang R, Qi J, Gao GF
Proc Natl Acad Sci U S A 114 E7564-E7573 (2017)
PMID: 28827346

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212919 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
MAb 4-5 heavy chain Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 24.7 KDa
Source organism: Homo sapiens
Expression system: Mammalia
Structure domains: Immunoglobulins
MAb 4-5 light chain Chain: B
Molecule details ›
Chain: B
Length: 222 amino acids
Theoretical weight: 24.39 KDa
Source organism: Homo sapiens
Expression system: Mammalia
Structure domains: Immunoglobulins
Glycoprotein N Chain: C
Molecule details ›
Chain: C
Length: 321 amino acids
Theoretical weight: 35.37 KDa
Source organism: Severe fever with thrombocytopenia syndrome virus
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: R4V2Q5 (Residues: 20-340; Coverage: 31%)
Sequence domains: Phlebovirus glycoprotein G1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 82.605Å b: 103.194Å c: 112.031Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.21
Expression systems:
  • Mammalia
  • Baculovirus expression vector pFastBac1-HM

Quick links

Citations

16 review citations

Common Features of Enveloped Viruses and Implications for Immunogen Design for Next-Generation Vaccines.
Rey et al. (2018)
15 more

9 mentions without citation

Antibodies to a Conserved Influenza Head Interface Epitope Protect by an IgG Subtype-Dependent Mechanism.
Watanabe et al. (2019)
8 more