› 5zsd

X-ray diffraction
2.6Å resolution

Crystal structure of monkey TLR7 in complex with IMDQ and GGUUGG

Released:
DOI: 10.2210/pdb5zsd/pdb
PDB entry 5zsd coloured by chain, front view.
PDB entry 5zsd coloured by chain, side view.
PDB entry 5zsd coloured by chain, top view.
Source organisms:
Primary publication:
Structural Analyses of Toll-like Receptor 7 Reveal Detailed RNA Sequence Specificity and Recognition Mechanism of Agonistic Ligands.
Zhang Z, Ohto U, Shibata T, Taoka M, Yamauchi Y, Sato R, Shukla NM, David SA, Isobe T, Miyake K, Shimizu T
Cell Rep 25 3371-3381.e5 (2018)
PMID: 30566863

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109973 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
TIR domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 823 amino acids
Theoretical weight: 94.75 KDa
Source organism: Macaca mulatta
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: B3Y653 (Residues: 27-839; Coverage: 78%)
Gene name: TLR7
Sequence domains: Leucine rich repeat
Structure domains: Ribonuclease Inhibitor
RNA (5'-R(P*UP*UP*GP*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 nucleotides
Theoretical weight: 1.93 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 11 x NAG
Ligand IDQ 2 x IDQ
Ligand SO4 14 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P212121
Unit cell:
a: 98.978Å b: 139.275Å c: 150.056Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.233
Expression systems:
  • Drosophila melanogaster
  • Not provided

Quick links

Citations

23 review citations

Thrombocytopathy and endotheliopathy: crucial contributors to COVID-19 thromboinflammation.
Gu et al. (2021)
22 more