› 5zsm

X-ray diffraction
2.5Å resolution

Crystal structure of monkey TLR7 in complex with GGUCCC

Released:
DOI: 10.2210/pdb5zsm/pdb
PDB entry 5zsm coloured by chain, front view.
PDB entry 5zsm coloured by chain, side view.
PDB entry 5zsm coloured by chain, top view.
Source organisms:
Primary publication:
Structural Analyses of Toll-like Receptor 7 Reveal Detailed RNA Sequence Specificity and Recognition Mechanism of Agonistic Ligands.
Zhang Z, Ohto U, Shibata T, Taoka M, Yamauchi Y, Sato R, Shukla NM, David SA, Isobe T, Miyake K, Shimizu T
Cell Rep 25 3371-3381.e5 (2018)
PMID: 30566863

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109976 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
TIR domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 823 amino acids
Theoretical weight: 94.75 KDa
Source organism: Macaca mulatta
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: B3Y653 (Residues: 27-839; Coverage: 78%)
Gene name: TLR7
Sequence domains: Leucine rich repeat
Structure domains: Ribonuclease Inhibitor
RNA (5'-R(*GP*UP*CP*CP*C)-3') Chains: D, E
Molecule details ›
Chains: D, E
Length: 6 nucleotides
Theoretical weight: 1.87 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 13 x NAG
Ligand SO4 9 x SO4
Ligand 9K9 2 x 9K9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 99.655Å b: 139.173Å c: 150.794Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.273
Expression systems:
  • Drosophila melanogaster
  • Not provided

Quick links

Citations

27 review citations

Thrombocytopathy and endotheliopathy: crucial contributors to COVID-19 thromboinflammation.
Gu et al. (2021)
26 more