› 5a50

X-ray diffraction
2.4Å resolution

The crystal structure of Arabidopsis thaliana CAR4 in complex with two calcium ions, Zn and Phopho Choline

Released:
DOI: 10.2210/pdb5a50/pdb
PDB entry 5a50 coloured by chain, front view.
PDB entry 5a50 coloured by chain, side view.
PDB entry 5a50 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Calcium-dependent oligomerization of CAR proteins at cell membrane modulates ABA signaling.
Diaz M, Sanchez-Barrena MJ, Gonzalez-Rubio JM, Rodriguez L, Fernandez D, Antoni R, Yunta C, Belda-Palazon B, Gonzalez-Guzman M, Peirats-Llobet M, Menendez M, Boskovic J, Marquez JA, Rodriguez PL, Albert A
Proc Natl Acad Sci U S A 113 E396-405 (2016)
PMID: 26719420

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192328 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein C2-DOMAIN ABA-RELATED 4 Chain: A
Molecule details ›
Chain: A
Length: 177 amino acids
Theoretical weight: 19.88 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9LVH4 (Residues: 1-177; Coverage: 100%)
Gene names: At3g17980, C2, CAR4, GAP1, MEB5.18
Sequence domains: C2 domain
Protein C2-DOMAIN ABA-RELATED 4 Chain: B
Molecule details ›
Chain: B
Length: 177 amino acids
Theoretical weight: 19.84 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9LVH4 (Residues: 1-177; Coverage: 100%)
Gene names: At3g17980, C2, CAR4, GAP1, MEB5.18
Sequence domains: C2 domain

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand PC 2 x PC
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 35.737Å b: 89.15Å c: 112.475Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.245
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Advances and current challenges in calcium signaling.
Kudla et al. (2018)
1 more

3 mentions without citation

Calcium-dependent oligomerization of CAR proteins at cell membrane modulates ABA signaling.
Diaz et al. (2016)
2 more