› 5a51

X-ray diffraction
1.6Å resolution

The crystal structure of Arabidopsis thaliana CAR4 in complex with two calcium ions and phophatidyl serine

Released:
DOI: 10.2210/pdb5a51/pdb
PDB entry 5a51 coloured by chain, front view.
PDB entry 5a51 coloured by chain, side view.
PDB entry 5a51 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Calcium-dependent oligomerization of CAR proteins at cell membrane modulates ABA signaling.
Diaz M, Sanchez-Barrena MJ, Gonzalez-Rubio JM, Rodriguez L, Fernandez D, Antoni R, Yunta C, Belda-Palazon B, Gonzalez-Guzman M, Peirats-Llobet M, Menendez M, Boskovic J, Marquez JA, Rodriguez PL, Albert A
Proc Natl Acad Sci U S A 113 E396-405 (2016)
PMID: 26719420

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein C2-DOMAIN ABA-RELATED 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 19.93 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9LVH4 (Residues: 1-177; Coverage: 100%)
Gene names: At3g17980, C2, CAR4, GAP1, MEB5.18
Sequence domains: C2 domain

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand SEP 1 x SEP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 35.583Å b: 89.769Å c: 109.642Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.222
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Advances and current challenges in calcium signaling.
Kudla et al. (2018)
1 more

2 mentions without citation

Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata.
Brault et al. (2019)
1 more