› 5a53

X-ray diffraction
2.4Å resolution

Crystal Structure of the Rpf2-Rrs1 complex

Released:
DOI: 10.2210/pdb5a53/pdb
PDB entry 5a53 coloured by chain, front view.
PDB entry 5a53 coloured by chain, side view.
PDB entry 5a53 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Chaperoning 5S RNA assembly.
Madru C, Lebaron S, Blaud M, Delbos L, Pipoli J, Pasmant E, Réty S, Leulliot N
OpenAccess logo Genes Dev 29 1432-46 (2015)
PMID: 26159998

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153141 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Regulator of ribosome biosynthesis Chain: A
Molecule details ›
Chain: A
Length: 65 amino acids
Theoretical weight: 7.27 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q08746 (Residues: 9-73; Coverage: 32%)
Gene names: RRS1, YOR294W
Sequence domains: Ribosome biogenesis regulatory protein (RRS1)
Regulator of ribosome biosynthesis Chain: B
Molecule details ›
Chain: B
Length: 22 amino acids
Theoretical weight: 2.46 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q08746 (Residues: 85-106; Coverage: 11%)
Gene names: RRS1, YOR294W
Ribosome biogenesis protein RPF2 Chain: C
Molecule details ›
Chain: C
Length: 230 amino acids
Theoretical weight: 26.58 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36160 (Residues: 23-252; Coverage: 67%)
Gene names: RPF2, YKR081C, YKR401
Sequence domains: Brix domain

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P43212
Unit cell:
a: 72.28Å b: 72.28Å c: 175.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Ribosome assembly coming into focus.
Klinge et al. (2019)
15 more

3 mentions without citation

Chaperoning 5S RNA assembly.
Madru et al. (2015)
2 more