› 5a6b

X-ray diffraction
1.77Å resolution

GH20C, Beta-hexosaminidase from Streptococcus pneumoniae in complex with PUGNAc

Released:
DOI: 10.2210/pdb5a6b/pdb
PDB entry 5a6b coloured by chain, front view.
PDB entry 5a6b coloured by chain, side view.
PDB entry 5a6b coloured by chain, top view.
Source organism: Streptococcus pneumoniae TIGR4
Primary publication:
A Second β-Hexosaminidase Encoded in the Streptococcus pneumoniae Genome Provides an Expanded Biochemical Ability to Degrade Host Glycans.
Robb M, Robb CS, Higgins MA, Hobbs JK, Paton JC, Boraston AB
J Biol Chem 290 30888-900 (2015)
PMID: 26491009

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-493566 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glycosyl hydrolase-related protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 649 amino acids
Theoretical weight: 74.13 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2US73 (Residues: 1-626; Coverage: 100%)
Gene name: SP_2141
Sequence domains:
Structure domains:
Glycosyl hydrolase-related protein Chain: D
Molecule details ›
Chain: D
Length: 648 amino acids
Theoretical weight: 74.02 KDa
Source organism: Streptococcus pneumoniae TIGR4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2US73 (Residues: 1-626; Coverage: 100%)
Gene name: SP_2141
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand OAN 4 x OAN
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 93.699Å b: 136.937Å c: 201.446Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Epidemiology, virulence factors and management of the pneumococcus.
Feldman et al. (2016)
2 more

1 mention without citation

A Second β-Hexosaminidase Encoded in the Streptococcus pneumoniae Genome Provides an Expanded Biochemical Ability to Degrade Host Glycans.
Robb et al. (2015)