5abb › Citations

Visualization of a polytopic membrane protein during SecY-mediated membrane insertion.

Bischoff L, Wickles S, Berninghausen O, van der Sluis EO, Beckmann R
Nat Commun 5 4103 (2014)
Cited: 42 times
EuropePMC logo PMID: 24912953

Abstract

The biogenesis of polytopic membrane proteins occurs co-translationally on ribosomes that are tightly bound to a membrane-embedded protein-conducting channel: the Sec-complex. The path that is followed by nascent proteins inside the ribosome and the Sec-complex is relatively well established; however, it is not clear what the fate of the N-terminal transmembrane domains (TMDs) of polytopic membrane proteins is when the C-terminal TMDs domains are not yet synthesized. Here, we present the sub-nanometer cryo-electron microscopy structure of an in vivo generated ribosome-SecY complex that carries a membrane insertion intermediate of proteorhodopsin (PR). The structure reveals a pre-opened Sec-complex and the first two TMDs of PR already outside the SecY complex directly in front of its proposed lateral gate. Thus, our structure is in agreement with positioning of N-terminal TMDs at the periphery of SecY, and in addition, it provides clues for the molecular mechanism underlying membrane protein topogenesis.

Reviews - 5abb mentioned but not cited (1)

  1. Bacterial Signal Peptides- Navigating the Journey of Proteins. Kaushik S, He H, Dalbey RE. Front Physiol 13 933153 (2022)


Reviews citing this publication (12)

  1. Protein export through the bacterial Sec pathway. Tsirigotaki A, De Geyter J, Šoštaric N, Economou A, Karamanou S. Nat Rev Microbiol 15 21-36 (2017)
  2. Mechanisms of integral membrane protein insertion and folding. Cymer F, von Heijne G, White SH. J Mol Biol 427 999-1022 (2015)
  3. Structural and Mechanistic Insights into Protein Translocation. Rapoport TA, Li L, Park E. Annu Rev Cell Dev Biol 33 369-390 (2017)
  4. Outer membrane protein folding from an energy landscape perspective. Schiffrin B, Brockwell DJ, Radford SE. BMC Biol 15 123 (2017)
  5. Highlighting membrane protein structure and function: A celebration of the Protein Data Bank. Li F, Egea PF, Vecchio AJ, Asial I, Gupta M, Paulino J, Bajaj R, Dickinson MS, Ferguson-Miller S, Monk BC, Stroud RM. J Biol Chem 296 100557 (2021)
  6. The Dynamic SecYEG Translocon. Oswald J, Njenga R, Natriashvili A, Sarmah P, Koch HG. Front Mol Biosci 8 664241 (2021)
  7. Structure-based working model of SecDF, a proton-driven bacterial protein translocation factor. Tsukazaki T. FEMS Microbiol Lett 365 (2018)
  8. The Molecular Biodiversity of Protein Targeting and Protein Transport Related to the Endoplasmic Reticulum. Tirincsi A, Sicking M, Hadzibeganovic D, Haßdenteufel S, Lang S. Int J Mol Sci 23 143 (2021)
  9. Structural Basis of the Sec Translocon and YidC Revealed Through X-ray Crystallography. Tsukazaki T. Protein J 38 249-261 (2019)
  10. Cotranslational Biogenesis of Membrane Proteins in Bacteria. Mercier E, Wang X, Bögeholz LAK, Wintermeyer W, Rodnina MV. Front Mol Biosci 9 871121 (2022)
  11. Sec translocon has an insertase-like function in addition to polypeptide conduction through the channel. Ito K, Shimokawa-Chiba N, Chiba S. F1000Res 8 F1000 Faculty Rev-2126 (2019)
  12. Coping with stress: How bacteria fine-tune protein synthesis and protein transport. Njenga R, Boele J, Öztürk Y, Koch HG. J Biol Chem 299 105163 (2023)

Articles citing this publication (29)



Quick links