› 5act

X-ray diffraction
1.81Å resolution

W228S-Investigation of the impact from residues W228 and Y233 in the metallo-beta-lactamase GIM-1

Released:
DOI: 10.2210/pdb5act/pdb
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PDB entry 5act coloured by chain, front view.
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PDB entry 5act coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Role of Residues W228 and Y233 in the Structure and Activity of Metallo-β-Lactamase GIM-1.
Skagseth S, Carlsen TJ, Bjerga GE, Spencer J, Samuelsen Ø, Leiros HK
Antimicrob Agents Chemother 60 990-1002 (2016)
PMID: 26643332

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180638 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 250 amino acids
Theoretical weight: 27.4 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q704V1 (Residues: 1-250; Coverage: 100%)
Gene names: blaGIM-1, blaGim-1
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand OCS 2 x OCS

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 38.644Å b: 133.007Å c: 40.86Å
α: 90° β: 95.28° γ: 90°
R-values:
R R work R free
0.182 0.179 0.23
Expression system: Escherichia coli BL21(DE3)

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Citations

5 citation in other articles

Metallo-β-lactamase inhibitors by bioisosteric replacement: Preparation, activity and binding.
Skagseth et al. (2017)
4 more

3 mentions without citation

Role of Residues W228 and Y233 in the Structure and Activity of Metallo-β-Lactamase GIM-1.
Skagseth et al. (2016)
2 more

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