› 5ad1

X-ray diffraction
1.32Å resolution

A complex of the synthetic siderophore analogue Fe(III)-8-LICAM with the CeuE periplasmic protein from Campylobacter jejuni

Released:
DOI: 10.2210/pdb5ad1/pdb
PDB entry 5ad1 coloured by chain, front view.
PDB entry 5ad1 coloured by chain, side view.
PDB entry 5ad1 coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Interactions of the periplasmic binding protein CeuE with Fe(III) n-LICAM4- siderophore analogues of varied linker length.
Wilde EJ, Hughes A, Blagova EV, Moroz OV, Thomas RP, Turkenburg JP, Raines DJ, Duhme-Klair AK, Wilson KS
OpenAccess logo Sci Rep 7 45941 (2017)
PMID: 28383577

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe/B12 periplasmic-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 32.02 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0P8Q4 (Residues: 44-330; Coverage: 93%)
Gene names: Cj1355, ceuE
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand 8LC 1 x 8LC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 42.98Å b: 55.98Å c: 140.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.14 0.181
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Iron Acquisition by Bacterial Pathogens: Beyond Tris-Catecholate Complexes.
Zhang et al. (2020)
1 more

1 mention without citation

Interactions of the periplasmic binding protein CeuE with Fe(III) n-LICAM4- siderophore analogues of varied linker length.
Wilde et al. (2017)