› 5agf

X-ray diffraction
1.09Å resolution

Nitrosyl complex of the D121Q variant of cytochrome c prime from Alcaligenes xylosoxidans

Released:
DOI: 10.2210/pdb5agf/pdb
PDB entry 5agf coloured by chain, front view.
PDB entry 5agf coloured by chain, side view.
PDB entry 5agf coloured by chain, top view.
Source organism: Achromobacter xylosoxidans
Primary publication:
Hydrogen bonding of the dissociated histidine ligand is not required for formation of a proximal NO adduct in cytochrome c'.
Ghafoor DD, Kekilli D, Abdullah GH, Dworkowski FS, Hassan HG, Wilson MT, Strange RW, Hough MA
J Biol Inorg Chem 20 949-56 (2015)
PMID: 26100643

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c' Chain: A
Molecule details ›
Chain: A
Length: 127 amino acids
Theoretical weight: 13.64 KDa
Source organism: Achromobacter xylosoxidans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00138 (Residues: 1-127; Coverage: 100%)
Sequence domains: Cytochrome C'
Structure domains: Cytochrome c/b562

Ligands and Environments

3 bound ligands:
Ligand HEC 1 x HEC
Ligand NO 1 x NO
Ligand SO4 1 x SO4
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6522
Unit cell:
a: 53.239Å b: 53.239Å c: 180.539Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.17 0.185
Expression system: Escherichia coli BL21(DE3)

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