› 5al6

X-ray diffraction
0.8Å resolution

Central Coiled-Coil Domain (CCCD) of Drosophila melanogaster Ana2. A natural, parallel, tetrameric coiled-coil bundle.

Released:
DOI: 10.2210/pdb5al6/pdb
PDB entry 5al6 coloured by chain, front view.
PDB entry 5al6 coloured by chain, side view.
PDB entry 5al6 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies.
Cottee MA, Muschalik N, Johnson S, Leveson J, Raff JW, Lea SM
OpenAccess logo Elife 4 e07236 (2015)
PMID: 26002084

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195058 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anastral spindle 2, isoform A Chain: A
Molecule details ›
Chain: A
Length: 49 amino acids
Theoretical weight: 5.64 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli B
UniProt:
  • Canonical: Q9XZ31 (Residues: 193-229; Coverage: 9%)
Gene names: Ana-2, Ana2, BcDNA:LD22033, CG8262, Dmel\CG8262, Dmel_CG8262, STIL, ana2

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I4
Unit cell:
a: 33.27Å b: 33.27Å c: 74.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.107 0.106 0.116
Expression system: Escherichia coli B

Quick links

Citations

11 review citations

Centrosome function and assembly in animal cells.
Conduit et al. (2015)
10 more

3 mentions without citation

The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies.
Cottee et al. (2015)
2 more