› 5awq

X-ray diffraction
1.48Å resolution

Arthrobacter globiformis T6 isomalto-dextranse complexed with panose

Released:
DOI: 10.2210/pdb5awq/pdb
PDB entry 5awq coloured by chain, front view.
PDB entry 5awq coloured by chain, side view.
PDB entry 5awq coloured by chain, top view.
Source organism: Arthrobacter globiformis
Primary publication:
Crystal Structure and Mutational Analysis of Isomalto-dextranase, a Member of Glycoside Hydrolase Family 27.
Okazawa Y, Miyazaki T, Yokoi G, Ishizaki Y, Nishikawa A, Tonozuka T
J Biol Chem 290 26339-49 (2015)
PMID: 26330557

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
CBM6 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 610 amino acids
Theoretical weight: 66.89 KDa
Source organism: Arthrobacter globiformis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7WSN5 (Residues: 31-636; Coverage: 100%)
Gene name: g2d
Sequence domains: Alpha galactosidase C-terminal beta sandwich domain

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand PO4 1 x PO4
1 modified residue:
Ligand SME 1 x SME

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: I41
Unit cell:
a: 123.878Å b: 123.878Å c: 85.261Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.187
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Review on recent advances in the properties, production and applications of microbial dextranases.
Mir et al. (2023)
9 other articles

2 mentions without citation

SIFTS: updated Structure Integration with Function, Taxonomy and Sequences resource allows 40-fold increase in coverage of structure-based annotations for proteins.
Dana et al. (2019)
1 more