› 5axi

X-ray diffraction
2.5Å resolution

Crystal structure of Cbl-b TKB domain in complex with Cblin

Released:
DOI: 10.2210/pdb5axi/pdb
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PDB entry 5axi coloured by chain, front view.
PDB entry 5axi coloured by chain, side view.
PDB entry 5axi coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural analysis of the TKB domain of ubiquitin ligase Cbl-b complexed with its small inhibitory peptide, Cblin.
Ohno A, Ochi A, Maita N, Ueji T, Bando A, Nakao R, Hirasaka K, Abe T, Teshima-Kondo S, Nemoto H, Okumura Y, Higashibata A, Yano S, Tochio H, Nikawa T
Arch Biochem Biophys 594 1-7 (2016)
PMID: 26874193

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152954 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase CBL-B Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 307 amino acids
Theoretical weight: 35.91 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3TTA7 (Residues: 38-343; Coverage: 31%)
Gene name: Cblb
Sequence domains:
Structure domains:
Insulin receptor substrate 1 Chain: E
Molecule details ›
Chain: E
Length: 5 amino acids
Theoretical weight: 662 Da
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P35569 (Residues: 606-610; Coverage: 0%)
Gene names: Irs-1, Irs1

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand CL 4 x CL
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 79.144Å b: 94.091Å c: 133.949Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.199 0.264
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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Citations

3 citation in other articles

In Vitro Ubiquitination Platform Identifies Methyl Ellipticiniums as Ubiquitin Ligase Inhibitors.
Wilson et al. (2021)
2 more

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