› 5axn

X-ray diffraction
2.7Å resolution

Crystal structure of Thg1 like protein (TLP) with tRNA(Phe) and GDPNP

Released:
DOI: 10.2210/pdb5axn/pdb
PDB entry 5axn coloured by chain, front view.
PDB entry 5axn coloured by chain, side view.
PDB entry 5axn coloured by chain, top view.
Source organisms:
Primary publication:
Template-dependent nucleotide addition in the reverse (3'-5') direction by Thg1-like protein.
Kimura S, Suzuki T, Chen M, Kato K, Yu J, Nakamura A, Tanaka I, Yao M
OpenAccess logo Sci Adv 2 e1501397 (2016)
PMID: 27051866

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103329 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
tRNAHis guanylyltransferase catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 251 amino acids
Theoretical weight: 29.33 KDa
Source organism: Methanosarcina acetivorans
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A1C7D1G9 (Residues: 1-251; Coverage: 100%)
Sequence domains: tRNAHis guanylyltransferase
Structure domains: Alpha-Beta Plaits
RNA (75-MER) Chain: P
Molecule details ›
Chain: P
Length: 75 nucleotides
Theoretical weight: 24.33 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
Sequence domains: tRNA

Ligands and Environments

2 bound ligands:
Ligand MG 5 x MG
Ligand GNP 2 x GNP
1 modified residue:
Ligand GTP 1 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: I222
Unit cell:
a: 82.255Å b: 134.052Å c: 147.384Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.225 0.267
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

2 review citations

The Role of 3' to 5' Reverse RNA Polymerization in tRNA Fidelity and Repair.
Chen et al. (2019)
1 more

3 mentions without citation

Template-dependent nucleotide addition in the reverse (3'-5') direction by Thg1-like protein.
Kimura et al. (2016)
2 more