› 5b0s

X-ray diffraction
2.1Å resolution

Beta-1,2-Mannobiose phosphorylase from Listeria innocua - beta-1,2-mannotriose complex

Released:
DOI: 10.2210/pdb5b0s/pdb
PDB entry 5b0s coloured by chain, front view.
PDB entry 5b0s coloured by chain, side view.
PDB entry 5b0s coloured by chain, top view.
Source organism: Listeria innocua Clip11262
Primary publication:
Characterization and crystal structure determination of β-1,2-mannobiose phosphorylase from Listeria innocua.
Tsuda T, Nihira T, Chiku K, Suzuki E, Arakawa T, Nishimoto M, Kitaoka M, Nakai H, Fushinobu S
FEBS Lett 589 3816-21 (2015)
PMID: 26632508

Function and Biology Details

Reaction catalysed: 
Beta-D-mannopyranosyl-(1->2)-D-mannopyranose + phosphate = D-mannopyranose + alpha-D-mannose 1-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-571762 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-1,2-mannobiose phosphorylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 363 amino acids
Theoretical weight: 41.11 KDa
Source organism: Listeria innocua Clip11262
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92DF6 (Residues: 1-355; Coverage: 100%)
Gene name: lin0857
Sequence domains: beta-1,4-mannooligosaccharide phosphorylase

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, BMA
Carbohydrate polymer
3 bound ligands:
Ligand SO4 9 x SO4
Ligand MES 3 x MES
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P3121
Unit cell:
a: 145.545Å b: 145.545Å c: 105.744Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.215
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Mannoside recognition and degradation by bacteria.
Ladevèze et al. (2017)
1 more