› 5b0w

X-ray diffraction
1.7Å resolution

Crystal structure of the 11-cis isomer of pharaonis halorhodopsin in the absence of halide ions

Released:
DOI: 10.2210/pdb5b0w/pdb
PDB entry 5b0w coloured by chain, front view.
PDB entry 5b0w coloured by chain, side view.
PDB entry 5b0w coloured by chain, top view.
Source organism: Natronomonas pharaonis DSM 2160
Primary publication:
Crystal Structure of the 11-cis Isomer of Pharaonis Halorhodopsin: Structural Constraints on Interconversions among Different Isomeric States.
Chan SK, Kawaguchi H, Kubo H, Murakami M, Ihara K, Maki K, Kouyama T
Biochemistry 55 4092-104 (2016)
PMID: 27352034

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165453 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Halorhodopsin Chains: A, B, D, E, F, G
Molecule details ›
Chains: A, B, D, E, F, G
Length: 291 amino acids
Theoretical weight: 30.98 KDa
Source organism: Natronomonas pharaonis DSM 2160
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

5 bound ligands:
Ligand RET 6 x RET
Ligand 22B 1 x 22B
Ligand L3P 6 x L3P
Ligand BNG 7 x BNG
Ligand ILE 1 x ILE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: C2
Unit cell:
a: 154.32Å b: 97.93Å c: 101.53Å
α: 90° β: 128.67° γ: 90°
R-values:
R R work R free
0.202 0.202 0.215

Quick links

Citations

5 citation in other articles

Applicability of Styrene-Maleic Acid Copolymer for Two Microbial Rhodopsins, RxR and HsSRI.
Ueta et al. (2020)
4 more

2 mentions without citation

Machine learning in computational modelling of membrane protein sequences and structures: From methodologies to applications.
Sun et al. (2023)
1 more