› 5b42

X-ray diffraction
1.35Å resolution

Crystal structure of the C-terminal endonuclease domain of Aquifex aeolicus MutL.

Released:
DOI: 10.2210/pdb5b42/pdb
PDB entry 5b42 coloured by chain, front view.
PDB entry 5b42 coloured by chain, side view.
PDB entry 5b42 coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Structural Features and Functional Dependency on β-Clamp Define Distinct Subfamilies of Bacterial Mismatch Repair Endonuclease MutL.
Fukui K, Baba S, Kumasaka T, Yano T
J Biol Chem 291 16990-7000 (2016)
PMID: 27369079

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA mismatch repair protein MutL Chain: A
Molecule details ›
Chain: A
Length: 102 amino acids
Theoretical weight: 12.35 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67518 (Residues: 324-425; Coverage: 24%)
Gene names: aq_1578, mutL
Sequence domains: MutL C terminal dimerisation domain
Structure domains: MutL, C-terminal domain, dimerisation subdomain

Ligands and Environments

2 bound ligands:
Ligand CD 6 x CD
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P43212
Unit cell:
a: 35.349Å b: 35.349Å c: 161.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.14 0.183
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Stochastic Processes and Component Plasticity Governing DNA Mismatch Repair.
Liu et al. (2018)
2 more

2 mentions without citation

Strand discrimination in DNA mismatch repair.
Putnam CD. (2021)
1 more