› 5b59

X-ray diffraction
2Å resolution

Hen egg-white lysozyme modified with a keto-ABNO.

Released:
DOI: 10.2210/pdb5b59/pdb
PDB entry 5b59 coloured by chain, front view.
PDB entry 5b59 coloured by chain, side view.
PDB entry 5b59 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Transition Metal-Free Tryptophan-Selective Bioconjugation of Proteins.
Seki Y, Ishiyama T, Sasaki D, Abe J, Sohma Y, Oisaki K, Kanai M
J Am Chem Soc 138 10798-801 (2016)
PMID: 27534812

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.35 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:
Ligand KTO 1 x KTO
1 modified residue:
Ligand TRO 1 x TRO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P43212
Unit cell:
a: 79.03Å b: 79.03Å c: 36.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.248

Quick links

Citations

13 review citations

Residue-Specific Peptide Modification: A Chemist's Guide.
deGruyter et al. (2017)
12 more

1 mention without citation

Developing informative microsatellite markers for non-model species using reference mapping against a model species' genome.
Hung et al. (2016)